Mass Spectrometry

3.0 1 Review SKU: LTMAS00CENDuration: 60 Minutes

This course describes the process of mass spectrometry and the analytical instrument that is used to perform this process. The focus is on a typical bench top gas mass/mass spectrometry (GC/MS) system. The system featured in this course includes a gas chromatograph with a capillary column, directly coupled to a mass spectrometer. The course explains the basic principles of mass spectral analysis, identifies the major parts of a typical GC/MS system, and describes the major steps of sample introduction, ionization (by electron-impact ionization), fragmentation, separation by mass (in a quadruple mass analyzer), detection of ions (by an electron multiplier), and data processing. The basic features of a typical mass spectrum and a typical total ion chromatogram are also described, and the use of the data system for controlling the GC/MS and for data processing, including performing library searches of mass spectra, is discussed.

Course Details

Specs

Training Time: 60 minutes

Compatibility: Desktop Only

Based on: Industry Standards and Best Practices

Languages: English

Learning Objectives

  • Define the following terms: "spectrum," "mass spectrometer," "mass spectrum," "abundance," "relative abundance," and "base ion."
  • Describe the basic process of mass spectrometry and explain why it can be used to perform qualitative and quantitative analyses.
  • List the major steps associated with the process of mass spectrometry.
  • Identify the major parts of a typical mass spectrometry system and describe the basic operation of the system.
  • State the separate purposes of gas chromatography and mass spectrometry in a GC/MS system.
  • List a major advantage and three limitations that relate to sample selection for a GC/MS system.
  • Describe a typical sample introduction step for a GC/MS system.
  • Describe the electron ionization process.
  • Describe how fragmentation occurs in a mass spectrometer.
  • Describe how a repeller and focusing optics can direct ions into a mass analyzer.
  • Describe how separation by mass occurs in a quadrupole mass analyzer.
  • Describe the use of an x-ray lens and an electron multiplier for ion collection, detection, and amplification.
  • Explain how the scan mode and the SIM mode enable a mass spectrometer to scan different ranges of ions in a mass spectrum.
  • Describe the data processing and control functions performed by the data system in a GC/MS system.
  • Define the following terms: "molecular ion," "parent ion," "isotopes," and "tuning."
  • Describe the features of a typical mass spectrum.
  • Describe the features of a total ion chromatogram.
  • Describe lab technician responsibilities associated with a GC/MS system.

Customer Reviews

5/29/2019

Good for Beginners

“It would be useful to talk about chemical ionization as well. Possibly issues that occur and troubleshooting maintenance.”

Alfred T. Verified Customer

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