High Pressure Liquid Chromatography

3.0 1 Review SKU: LTHIP00CENDuration: 60 Minutes

This course discusses the separation of sample components by the process of high pressure liquid chromatography (HPLC). It explains the basic principles of the HPLC process and describes the major parts of a typical HPLC system, including a solvent pumping system, an auto sampler, a column, a variable wavelength UV detector, and a data system. It describes the following major steps for performing an HPLC analysis: solvent preparation and delivery; sample preparation and injection; sample separation; component detection; and data processing. It also discusses how information presented on an HPLC chromatogram may be used for qualitative and quantitative analyses.

Course Details


Training Time: 60 minutes

Compatibility: Desktop Only

Based on: Industry Standards and Best Practices

Languages: English

Learning Objectives

  • Define the following terms: "gas chromatography," "liquid chromatography," "solvent," and "HPLC."
  • List the three main goals of HPLC.
  • Identify the major parts of a typical HPLC system and describe the system's basic operation.
  • Identify types of liquid chromatography that may be performed with an HPLC system.
  • Describe equipment and materials involved in the preparation of the sample, the solvent, and the pump for a typical HPLC system.
  • Describe the basic features and operation of a typical auto sampler for an HPLC system.
  • Describe the function and features of a typical HPLC column.
  • Describe the function and features of a typical pre-column and a typical guard column.
  • Define the following terms: "normal phase liquid chromatography," "reversed phase liquid chromatography," "dead zone," "dead volume," "isocratic elution," and "gradient elution."
  • Describe the basic features and function of a typical low pressure gradient formation system.
  • Describe the features and the basic operation of a typical variable wavelength UV detector.
  • List functions that are typically performed by a data system.
  • Describe in general terms how an HPLC chromatogram provides information for qualitative and quantitative analyses.

Customer Reviews


Want More Info About Columns

“This needed to talk more about columns, why certain solvents are chosen, and other types of detectors.”

Alfred T. Verified Customer

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